xrcc5 antibody Search Results


ku80  (Novus Biologicals)
92
Novus Biologicals ku80
A , oviductal telomere length in adult female rats exposed to gestational hypoxia compared to normoxia. B , effect of gestational hypoxia compared to normoxia on gene expression of components ( Ku70 and <t>Ku80</t> ) of the DNA‐activated protein kinase (DNA‐PK) in the oviducts. C , effect of gestational hypoxia compared to normoxia on protein expression of KU70 and KU80. Data shown as the mean ± SEM. Open bars: normoxia (21% oxygen) during gestation, grey bars: hypoxia (13% oxygen) during gestation. * P < 0.05, *** P < 0.001. n = 7–8 for all groups ( n refers to the number of litters)
Ku80, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 92 stars, based on 1 article reviews
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anti human ku80  (R&D Systems)
94
R&D Systems anti human ku80
A , oviductal telomere length in adult female rats exposed to gestational hypoxia compared to normoxia. B , effect of gestational hypoxia compared to normoxia on gene expression of components ( Ku70 and <t>Ku80</t> ) of the DNA‐activated protein kinase (DNA‐PK) in the oviducts. C , effect of gestational hypoxia compared to normoxia on protein expression of KU70 and KU80. Data shown as the mean ± SEM. Open bars: normoxia (21% oxygen) during gestation, grey bars: hypoxia (13% oxygen) during gestation. * P < 0.05, *** P < 0.001. n = 7–8 for all groups ( n refers to the number of litters)
Anti Human Ku80, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
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xrcc5  (Proteintech)
94
Proteintech xrcc5
Primers used in this study.
Xrcc5, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
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ku80  (Proteintech)
93
Proteintech ku80
Primers used in this study.
Ku80, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
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antibodies to ku80 pb9464  (Boster Bio)
92
Boster Bio antibodies to ku80 pb9464
Fig. 5. Quercetin inhibited NHEJ and HR pathways phosphorylation. Total proteins lysis and extraction after A549 and H1299 cultured with 0, 12.5, 50, and 200 μM quercetin for 24 h. In NHEJ pathways (A) the expression of p-DNA-PKcsS2056 (B, C), KU70 (D, E) and <t>KU80</t> (F, G), and in HR pathways (H) the phosphorylation of p- ATRS428 (I, J), p-Chek1S345 (K, L), p-ATMS1981 (M, N) and Chek2T68 (O, P) were detected by western blot in both A549 and H1299 cells. And the results were measured by ImageJ and expressed as protein expression relative to GAPDH (mean ± S.D., n = 3). #p > 0.05, *p < 0.05, * *p < 0.01, * **p < 0.001 relative to values in the respective 0 μM group (B, p = 0.0048, R2 =0.7844, F=9.700; C, p = 0.0002, R2 =0.9042, F=25.17; D, p = 0.0054, R2 =0.7782, F=9.358; E, p = 0.0045, R2 =0.7879, F=9.908; F, p = 0.0037, R2 =0.7990, F=10.60; G, p = 0.0016, R2 =0.8379, F=13.79; I, p = 0.0034, R2 =0.8028, F=10.85; J, p = 0.0032, R2 =0.8066, F=11.12; K, p = 0.0010, R2 =0.8564, F=15.91; L, p = 0.0016, R2 =0.8379, F=13.79; M, p = 0.0044, R2 =0.7898, F=10.02; N, p = 0.0065, R2 =0.7676, F=8.806; O, p = 0.0030, R2 =0.0.8087, F=11.27; P, p = 0.0026, R2 =0.8166, F=11.87), One-way ANOVA test. The mRNA in A549 (Q) and H1299 (R) cells was extracted and reverse transcribed to cDNA for RT-qPCR analysis (mean ± S.D., n = 3). #p > 0.05, *p < 0.05, * *p < 0.01, * **p < 0.001, * ** *p < 0.0001 relative to values in the respective 0 μM group, One-way ANOVA test.
Antibodies To Ku80 Pb9464, supplied by Boster Bio, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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monoclonal antibody  (Boster Bio)
90
Boster Bio monoclonal antibody
Fig. 5. Quercetin inhibited NHEJ and HR pathways phosphorylation. Total proteins lysis and extraction after A549 and H1299 cultured with 0, 12.5, 50, and 200 μM quercetin for 24 h. In NHEJ pathways (A) the expression of p-DNA-PKcsS2056 (B, C), KU70 (D, E) and <t>KU80</t> (F, G), and in HR pathways (H) the phosphorylation of p- ATRS428 (I, J), p-Chek1S345 (K, L), p-ATMS1981 (M, N) and Chek2T68 (O, P) were detected by western blot in both A549 and H1299 cells. And the results were measured by ImageJ and expressed as protein expression relative to GAPDH (mean ± S.D., n = 3). #p > 0.05, *p < 0.05, * *p < 0.01, * **p < 0.001 relative to values in the respective 0 μM group (B, p = 0.0048, R2 =0.7844, F=9.700; C, p = 0.0002, R2 =0.9042, F=25.17; D, p = 0.0054, R2 =0.7782, F=9.358; E, p = 0.0045, R2 =0.7879, F=9.908; F, p = 0.0037, R2 =0.7990, F=10.60; G, p = 0.0016, R2 =0.8379, F=13.79; I, p = 0.0034, R2 =0.8028, F=10.85; J, p = 0.0032, R2 =0.8066, F=11.12; K, p = 0.0010, R2 =0.8564, F=15.91; L, p = 0.0016, R2 =0.8379, F=13.79; M, p = 0.0044, R2 =0.7898, F=10.02; N, p = 0.0065, R2 =0.7676, F=8.806; O, p = 0.0030, R2 =0.0.8087, F=11.27; P, p = 0.0026, R2 =0.8166, F=11.87), One-way ANOVA test. The mRNA in A549 (Q) and H1299 (R) cells was extracted and reverse transcribed to cDNA for RT-qPCR analysis (mean ± S.D., n = 3). #p > 0.05, *p < 0.05, * *p < 0.01, * **p < 0.001, * ** *p < 0.0001 relative to values in the respective 0 μM group, One-way ANOVA test.
Monoclonal Antibody, supplied by Boster Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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monoclonal antibody - by Bioz Stars, 2026-03
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rabbit secondary antibodies  (Boster Bio)
93
Boster Bio rabbit secondary antibodies
Fig. 5. Quercetin inhibited NHEJ and HR pathways phosphorylation. Total proteins lysis and extraction after A549 and H1299 cultured with 0, 12.5, 50, and 200 μM quercetin for 24 h. In NHEJ pathways (A) the expression of p-DNA-PKcsS2056 (B, C), KU70 (D, E) and <t>KU80</t> (F, G), and in HR pathways (H) the phosphorylation of p- ATRS428 (I, J), p-Chek1S345 (K, L), p-ATMS1981 (M, N) and Chek2T68 (O, P) were detected by western blot in both A549 and H1299 cells. And the results were measured by ImageJ and expressed as protein expression relative to GAPDH (mean ± S.D., n = 3). #p > 0.05, *p < 0.05, * *p < 0.01, * **p < 0.001 relative to values in the respective 0 μM group (B, p = 0.0048, R2 =0.7844, F=9.700; C, p = 0.0002, R2 =0.9042, F=25.17; D, p = 0.0054, R2 =0.7782, F=9.358; E, p = 0.0045, R2 =0.7879, F=9.908; F, p = 0.0037, R2 =0.7990, F=10.60; G, p = 0.0016, R2 =0.8379, F=13.79; I, p = 0.0034, R2 =0.8028, F=10.85; J, p = 0.0032, R2 =0.8066, F=11.12; K, p = 0.0010, R2 =0.8564, F=15.91; L, p = 0.0016, R2 =0.8379, F=13.79; M, p = 0.0044, R2 =0.7898, F=10.02; N, p = 0.0065, R2 =0.7676, F=8.806; O, p = 0.0030, R2 =0.0.8087, F=11.27; P, p = 0.0026, R2 =0.8166, F=11.87), One-way ANOVA test. The mRNA in A549 (Q) and H1299 (R) cells was extracted and reverse transcribed to cDNA for RT-qPCR analysis (mean ± S.D., n = 3). #p > 0.05, *p < 0.05, * *p < 0.01, * **p < 0.001, * ** *p < 0.0001 relative to values in the respective 0 μM group, One-way ANOVA test.
Rabbit Secondary Antibodies, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
rabbit secondary antibodies - by Bioz Stars, 2026-03
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ku80 antibody  (R&D Systems)
93
R&D Systems ku80 antibody
Fig. 5. Quercetin inhibited NHEJ and HR pathways phosphorylation. Total proteins lysis and extraction after A549 and H1299 cultured with 0, 12.5, 50, and 200 μM quercetin for 24 h. In NHEJ pathways (A) the expression of p-DNA-PKcsS2056 (B, C), KU70 (D, E) and <t>KU80</t> (F, G), and in HR pathways (H) the phosphorylation of p- ATRS428 (I, J), p-Chek1S345 (K, L), p-ATMS1981 (M, N) and Chek2T68 (O, P) were detected by western blot in both A549 and H1299 cells. And the results were measured by ImageJ and expressed as protein expression relative to GAPDH (mean ± S.D., n = 3). #p > 0.05, *p < 0.05, * *p < 0.01, * **p < 0.001 relative to values in the respective 0 μM group (B, p = 0.0048, R2 =0.7844, F=9.700; C, p = 0.0002, R2 =0.9042, F=25.17; D, p = 0.0054, R2 =0.7782, F=9.358; E, p = 0.0045, R2 =0.7879, F=9.908; F, p = 0.0037, R2 =0.7990, F=10.60; G, p = 0.0016, R2 =0.8379, F=13.79; I, p = 0.0034, R2 =0.8028, F=10.85; J, p = 0.0032, R2 =0.8066, F=11.12; K, p = 0.0010, R2 =0.8564, F=15.91; L, p = 0.0016, R2 =0.8379, F=13.79; M, p = 0.0044, R2 =0.7898, F=10.02; N, p = 0.0065, R2 =0.7676, F=8.806; O, p = 0.0030, R2 =0.0.8087, F=11.27; P, p = 0.0026, R2 =0.8166, F=11.87), One-way ANOVA test. The mRNA in A549 (Q) and H1299 (R) cells was extracted and reverse transcribed to cDNA for RT-qPCR analysis (mean ± S.D., n = 3). #p > 0.05, *p < 0.05, * *p < 0.01, * **p < 0.001, * ** *p < 0.0001 relative to values in the respective 0 μM group, One-way ANOVA test.
Ku80 Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ku80 antibody/product/R&D Systems
Average 93 stars, based on 1 article reviews
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primary antibody against xrcc5 genetex clone s10b1  (GeneTex)
90
GeneTex primary antibody against xrcc5 genetex clone s10b1
Fig. 5. Quercetin inhibited NHEJ and HR pathways phosphorylation. Total proteins lysis and extraction after A549 and H1299 cultured with 0, 12.5, 50, and 200 μM quercetin for 24 h. In NHEJ pathways (A) the expression of p-DNA-PKcsS2056 (B, C), KU70 (D, E) and <t>KU80</t> (F, G), and in HR pathways (H) the phosphorylation of p- ATRS428 (I, J), p-Chek1S345 (K, L), p-ATMS1981 (M, N) and Chek2T68 (O, P) were detected by western blot in both A549 and H1299 cells. And the results were measured by ImageJ and expressed as protein expression relative to GAPDH (mean ± S.D., n = 3). #p > 0.05, *p < 0.05, * *p < 0.01, * **p < 0.001 relative to values in the respective 0 μM group (B, p = 0.0048, R2 =0.7844, F=9.700; C, p = 0.0002, R2 =0.9042, F=25.17; D, p = 0.0054, R2 =0.7782, F=9.358; E, p = 0.0045, R2 =0.7879, F=9.908; F, p = 0.0037, R2 =0.7990, F=10.60; G, p = 0.0016, R2 =0.8379, F=13.79; I, p = 0.0034, R2 =0.8028, F=10.85; J, p = 0.0032, R2 =0.8066, F=11.12; K, p = 0.0010, R2 =0.8564, F=15.91; L, p = 0.0016, R2 =0.8379, F=13.79; M, p = 0.0044, R2 =0.7898, F=10.02; N, p = 0.0065, R2 =0.7676, F=8.806; O, p = 0.0030, R2 =0.0.8087, F=11.27; P, p = 0.0026, R2 =0.8166, F=11.87), One-way ANOVA test. The mRNA in A549 (Q) and H1299 (R) cells was extracted and reverse transcribed to cDNA for RT-qPCR analysis (mean ± S.D., n = 3). #p > 0.05, *p < 0.05, * *p < 0.01, * **p < 0.001, * ** *p < 0.0001 relative to values in the respective 0 μM group, One-way ANOVA test.
Primary Antibody Against Xrcc5 Genetex Clone S10b1, supplied by GeneTex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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xrcc5 antibody  (Beijing Solarbio Science)
90
Beijing Solarbio Science xrcc5 antibody
<t>XRCC5</t> was a target of miR‐326. (a, b) The expression of candidate targets was tested by quantitative reverse transcription‐polymerase chain reaction (qRT‐PCR) in breast cancer (BC) cells transfected with inhibitor NC or miR‐326 inhibitor. (c) The binding sites between miR‐326 and XRCC5 3′UTR predicted by TargetScan online software are shown. (d, e) Dual‐luciferase reporter assay was used to confirm the interaction between miR‐326 and XRCC5 3′UTR. (f) The mRNA expression of XRCC5 in BC tissues and adjacent normal tissues was examined by qRT‐PCR. (g) The protein expression of XRCC5 in MCF‐10A cells and both BC cells was determined using western blot (WB) analysis. (h) XRCC5 protein expression was tested by WB analysis in BC cells transfected with si‐NC, si‐RUSC1‐AS1, si‐RUSC1‐AS1 + inhibitor NC or si‐RUSC1‐AS1 + miR‐326 inhibitor. (i) The transfection efficiency of pc‐XRCC5 was assessed by measuring XRCC5 protein expression using WB analysis. (j) BC cells were transfected with miRNA NC, miR‐326 mimic, miR‐326 mimic + pc‐NC or miR‐326 mimic + pc‐XRCC5. WB analysis was used to detect XRCC5 protein expression. * p < 0.05.
Xrcc5 Antibody, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-ku86 ab a5862  (ABclonal Biotechnology)
90
ABclonal Biotechnology anti-ku86 ab a5862
<t>XRCC5</t> was a target of miR‐326. (a, b) The expression of candidate targets was tested by quantitative reverse transcription‐polymerase chain reaction (qRT‐PCR) in breast cancer (BC) cells transfected with inhibitor NC or miR‐326 inhibitor. (c) The binding sites between miR‐326 and XRCC5 3′UTR predicted by TargetScan online software are shown. (d, e) Dual‐luciferase reporter assay was used to confirm the interaction between miR‐326 and XRCC5 3′UTR. (f) The mRNA expression of XRCC5 in BC tissues and adjacent normal tissues was examined by qRT‐PCR. (g) The protein expression of XRCC5 in MCF‐10A cells and both BC cells was determined using western blot (WB) analysis. (h) XRCC5 protein expression was tested by WB analysis in BC cells transfected with si‐NC, si‐RUSC1‐AS1, si‐RUSC1‐AS1 + inhibitor NC or si‐RUSC1‐AS1 + miR‐326 inhibitor. (i) The transfection efficiency of pc‐XRCC5 was assessed by measuring XRCC5 protein expression using WB analysis. (j) BC cells were transfected with miRNA NC, miR‐326 mimic, miR‐326 mimic + pc‐NC or miR‐326 mimic + pc‐XRCC5. WB analysis was used to detect XRCC5 protein expression. * p < 0.05.
Anti Ku86 Ab A5862, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ku80/xrcc5 antibody (3d8)  (Bio-Techne corporation)
90
Bio-Techne corporation ku80/xrcc5 antibody (3d8)
<t>XRCC5</t> was a target of miR‐326. (a, b) The expression of candidate targets was tested by quantitative reverse transcription‐polymerase chain reaction (qRT‐PCR) in breast cancer (BC) cells transfected with inhibitor NC or miR‐326 inhibitor. (c) The binding sites between miR‐326 and XRCC5 3′UTR predicted by TargetScan online software are shown. (d, e) Dual‐luciferase reporter assay was used to confirm the interaction between miR‐326 and XRCC5 3′UTR. (f) The mRNA expression of XRCC5 in BC tissues and adjacent normal tissues was examined by qRT‐PCR. (g) The protein expression of XRCC5 in MCF‐10A cells and both BC cells was determined using western blot (WB) analysis. (h) XRCC5 protein expression was tested by WB analysis in BC cells transfected with si‐NC, si‐RUSC1‐AS1, si‐RUSC1‐AS1 + inhibitor NC or si‐RUSC1‐AS1 + miR‐326 inhibitor. (i) The transfection efficiency of pc‐XRCC5 was assessed by measuring XRCC5 protein expression using WB analysis. (j) BC cells were transfected with miRNA NC, miR‐326 mimic, miR‐326 mimic + pc‐NC or miR‐326 mimic + pc‐XRCC5. WB analysis was used to detect XRCC5 protein expression. * p < 0.05.
Ku80/Xrcc5 Antibody (3d8), supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


A , oviductal telomere length in adult female rats exposed to gestational hypoxia compared to normoxia. B , effect of gestational hypoxia compared to normoxia on gene expression of components ( Ku70 and Ku80 ) of the DNA‐activated protein kinase (DNA‐PK) in the oviducts. C , effect of gestational hypoxia compared to normoxia on protein expression of KU70 and KU80. Data shown as the mean ± SEM. Open bars: normoxia (21% oxygen) during gestation, grey bars: hypoxia (13% oxygen) during gestation. * P < 0.05, *** P < 0.001. n = 7–8 for all groups ( n refers to the number of litters)

Journal: The Journal of Physiology

Article Title: Chronic fetal hypoxia disrupts the peri‐conceptual environment in next‐generation adult female rats

doi: 10.1113/JP277431

Figure Lengend Snippet: A , oviductal telomere length in adult female rats exposed to gestational hypoxia compared to normoxia. B , effect of gestational hypoxia compared to normoxia on gene expression of components ( Ku70 and Ku80 ) of the DNA‐activated protein kinase (DNA‐PK) in the oviducts. C , effect of gestational hypoxia compared to normoxia on protein expression of KU70 and KU80. Data shown as the mean ± SEM. Open bars: normoxia (21% oxygen) during gestation, grey bars: hypoxia (13% oxygen) during gestation. * P < 0.05, *** P < 0.001. n = 7–8 for all groups ( n refers to the number of litters)

Article Snippet: Detection steps used the following primary antibodies: P53 (R&D Systems, R&D Systems, Minneapolis, MN, USA; catalogue no. MAB1355; dilution 1:1000; RRID:AB_357649), P16 INK (Abcam, Cambridge, UK; catalogue no. Ab189034; dilution 1:1000; RRID:AB_2737282), OGG1 (Novus Biologicals, Littleton, CO, USA; catalogue no. NB100‐106; dilution 1:1000; RRID:AB_10104097), MRE11 (ProteinTech, Cambridge, UK; catalogue no. 10744‐1‐AP; dilution 1:1000; RRID:AB2145118), KU70 (ProteinTech; catalogue no. 10723‐1‐AP; dilution 1:1000; RRID:AB_), KU80 (Novus Biologicals; catalogue no. NB100‐508; dilution 1:1000; RRID:AB_2218756), Total Ox Phos rodent antibody cocktail (Abcam; catalogue no. Ab110413; dilution 1:5000; RRID:AB_2629281), HIF1α (Abcam; catalogue no. Ab51608; dilution 1:1000; RRID:AB_880418), GP91 phox (ProteinTech; catalogue no. 19013‐1‐AP; RRID:AB_1342287; dilution 1:1000), P47 phox (ProteinTech; catalogue no. 15551‐1‐AP; dilution 1:1000; RRID:AB_11182937), XO (Santa‐Cruz, Wimbledon, UK; catalogue no. SC‐20991; dilution 1:200, RRID:AB_2214858), HMOX1 (ProteinTech; catalogue no. 20960‐1‐AP; dilution 1:1000; RRID:AB_10732601), Catalase (Abcam; catalogue no. Ab1877‐10; dilution 1:10,000; RRID:AB_187710), MnSOD (Upstate, Watford, UK; catalogue no. 06‐984; RRID:AB_310325; dilution 1:1000), CuZnSOD (ProteinTech; catalogue no. 10269‐1‐AP; dilution 1:1000; RRID:AB_2193750).

Techniques: Gene Expression, Expressing

Effect of gestational hypoxia compared to normoxia on gene expression in the oviducts of adult female rats

Journal: The Journal of Physiology

Article Title: Chronic fetal hypoxia disrupts the peri‐conceptual environment in next‐generation adult female rats

doi: 10.1113/JP277431

Figure Lengend Snippet: Effect of gestational hypoxia compared to normoxia on gene expression in the oviducts of adult female rats

Article Snippet: Detection steps used the following primary antibodies: P53 (R&D Systems, R&D Systems, Minneapolis, MN, USA; catalogue no. MAB1355; dilution 1:1000; RRID:AB_357649), P16 INK (Abcam, Cambridge, UK; catalogue no. Ab189034; dilution 1:1000; RRID:AB_2737282), OGG1 (Novus Biologicals, Littleton, CO, USA; catalogue no. NB100‐106; dilution 1:1000; RRID:AB_10104097), MRE11 (ProteinTech, Cambridge, UK; catalogue no. 10744‐1‐AP; dilution 1:1000; RRID:AB2145118), KU70 (ProteinTech; catalogue no. 10723‐1‐AP; dilution 1:1000; RRID:AB_), KU80 (Novus Biologicals; catalogue no. NB100‐508; dilution 1:1000; RRID:AB_2218756), Total Ox Phos rodent antibody cocktail (Abcam; catalogue no. Ab110413; dilution 1:5000; RRID:AB_2629281), HIF1α (Abcam; catalogue no. Ab51608; dilution 1:1000; RRID:AB_880418), GP91 phox (ProteinTech; catalogue no. 19013‐1‐AP; RRID:AB_1342287; dilution 1:1000), P47 phox (ProteinTech; catalogue no. 15551‐1‐AP; dilution 1:1000; RRID:AB_11182937), XO (Santa‐Cruz, Wimbledon, UK; catalogue no. SC‐20991; dilution 1:200, RRID:AB_2214858), HMOX1 (ProteinTech; catalogue no. 20960‐1‐AP; dilution 1:1000; RRID:AB_10732601), Catalase (Abcam; catalogue no. Ab1877‐10; dilution 1:10,000; RRID:AB_187710), MnSOD (Upstate, Watford, UK; catalogue no. 06‐984; RRID:AB_310325; dilution 1:1000), CuZnSOD (ProteinTech; catalogue no. 10269‐1‐AP; dilution 1:1000; RRID:AB_2193750).

Techniques: Gene Expression

Effect of gestational hypoxia compared to normoxia on protein expression in the oviducts of adult female rats

Journal: The Journal of Physiology

Article Title: Chronic fetal hypoxia disrupts the peri‐conceptual environment in next‐generation adult female rats

doi: 10.1113/JP277431

Figure Lengend Snippet: Effect of gestational hypoxia compared to normoxia on protein expression in the oviducts of adult female rats

Article Snippet: Detection steps used the following primary antibodies: P53 (R&D Systems, R&D Systems, Minneapolis, MN, USA; catalogue no. MAB1355; dilution 1:1000; RRID:AB_357649), P16 INK (Abcam, Cambridge, UK; catalogue no. Ab189034; dilution 1:1000; RRID:AB_2737282), OGG1 (Novus Biologicals, Littleton, CO, USA; catalogue no. NB100‐106; dilution 1:1000; RRID:AB_10104097), MRE11 (ProteinTech, Cambridge, UK; catalogue no. 10744‐1‐AP; dilution 1:1000; RRID:AB2145118), KU70 (ProteinTech; catalogue no. 10723‐1‐AP; dilution 1:1000; RRID:AB_), KU80 (Novus Biologicals; catalogue no. NB100‐508; dilution 1:1000; RRID:AB_2218756), Total Ox Phos rodent antibody cocktail (Abcam; catalogue no. Ab110413; dilution 1:5000; RRID:AB_2629281), HIF1α (Abcam; catalogue no. Ab51608; dilution 1:1000; RRID:AB_880418), GP91 phox (ProteinTech; catalogue no. 19013‐1‐AP; RRID:AB_1342287; dilution 1:1000), P47 phox (ProteinTech; catalogue no. 15551‐1‐AP; dilution 1:1000; RRID:AB_11182937), XO (Santa‐Cruz, Wimbledon, UK; catalogue no. SC‐20991; dilution 1:200, RRID:AB_2214858), HMOX1 (ProteinTech; catalogue no. 20960‐1‐AP; dilution 1:1000; RRID:AB_10732601), Catalase (Abcam; catalogue no. Ab1877‐10; dilution 1:10,000; RRID:AB_187710), MnSOD (Upstate, Watford, UK; catalogue no. 06‐984; RRID:AB_310325; dilution 1:1000), CuZnSOD (ProteinTech; catalogue no. 10269‐1‐AP; dilution 1:1000; RRID:AB_2193750).

Techniques: Expressing

Primers used in this study.

Journal: Viruses

Article Title: Proteomic Analysis of Vero Cells Infected with Pseudorabies Virus

doi: 10.3390/v14040755

Figure Lengend Snippet: Primers used in this study.

Article Snippet: The primary antibodies used in this study were specific for XRCC5 (16389-1-AP, Proteintech, Rosemont, IL, USA), XRCC6 (10723-1-AP, Proteintech, Rosemont, IL, USA), β-actin (66009-1-Ig, Proteintech, Rosemont, IL, USA), VP5 (prepared in our lab), and gB (prepared in our lab).

Techniques: Sequencing

Validation of proteomics data by western blot and RT-qPCR. ( A ) Vero cells infected with PRV for 24 h were collected and western blot was performed to detect the expression of XRCC5 and XRCC6 with corresponding antibodies. ( B ) The western blot and proteomics ratio of XRCC5 and XRCC6. ( C ) Relative XRCC6 transcription in Vero cells. ( D ) Relative XRCC5 transcription in Vero cells. ( E ) The expression of XRCC5 and XRCC6 in PK-15 infected with PRV. ( F ) The expression of XRCC5 and XRCC6 in CRL-2843 infected with PRV. ** indicates significance at a 99% confidence interval ( p < 0.01).

Journal: Viruses

Article Title: Proteomic Analysis of Vero Cells Infected with Pseudorabies Virus

doi: 10.3390/v14040755

Figure Lengend Snippet: Validation of proteomics data by western blot and RT-qPCR. ( A ) Vero cells infected with PRV for 24 h were collected and western blot was performed to detect the expression of XRCC5 and XRCC6 with corresponding antibodies. ( B ) The western blot and proteomics ratio of XRCC5 and XRCC6. ( C ) Relative XRCC6 transcription in Vero cells. ( D ) Relative XRCC5 transcription in Vero cells. ( E ) The expression of XRCC5 and XRCC6 in PK-15 infected with PRV. ( F ) The expression of XRCC5 and XRCC6 in CRL-2843 infected with PRV. ** indicates significance at a 99% confidence interval ( p < 0.01).

Article Snippet: The primary antibodies used in this study were specific for XRCC5 (16389-1-AP, Proteintech, Rosemont, IL, USA), XRCC6 (10723-1-AP, Proteintech, Rosemont, IL, USA), β-actin (66009-1-Ig, Proteintech, Rosemont, IL, USA), VP5 (prepared in our lab), and gB (prepared in our lab).

Techniques: Biomarker Discovery, Western Blot, Quantitative RT-PCR, Infection, Expressing

Fig. 5. Quercetin inhibited NHEJ and HR pathways phosphorylation. Total proteins lysis and extraction after A549 and H1299 cultured with 0, 12.5, 50, and 200 μM quercetin for 24 h. In NHEJ pathways (A) the expression of p-DNA-PKcsS2056 (B, C), KU70 (D, E) and KU80 (F, G), and in HR pathways (H) the phosphorylation of p- ATRS428 (I, J), p-Chek1S345 (K, L), p-ATMS1981 (M, N) and Chek2T68 (O, P) were detected by western blot in both A549 and H1299 cells. And the results were measured by ImageJ and expressed as protein expression relative to GAPDH (mean ± S.D., n = 3). #p > 0.05, *p < 0.05, * *p < 0.01, * **p < 0.001 relative to values in the respective 0 μM group (B, p = 0.0048, R2 =0.7844, F=9.700; C, p = 0.0002, R2 =0.9042, F=25.17; D, p = 0.0054, R2 =0.7782, F=9.358; E, p = 0.0045, R2 =0.7879, F=9.908; F, p = 0.0037, R2 =0.7990, F=10.60; G, p = 0.0016, R2 =0.8379, F=13.79; I, p = 0.0034, R2 =0.8028, F=10.85; J, p = 0.0032, R2 =0.8066, F=11.12; K, p = 0.0010, R2 =0.8564, F=15.91; L, p = 0.0016, R2 =0.8379, F=13.79; M, p = 0.0044, R2 =0.7898, F=10.02; N, p = 0.0065, R2 =0.7676, F=8.806; O, p = 0.0030, R2 =0.0.8087, F=11.27; P, p = 0.0026, R2 =0.8166, F=11.87), One-way ANOVA test. The mRNA in A549 (Q) and H1299 (R) cells was extracted and reverse transcribed to cDNA for RT-qPCR analysis (mean ± S.D., n = 3). #p > 0.05, *p < 0.05, * *p < 0.01, * **p < 0.001, * ** *p < 0.0001 relative to values in the respective 0 μM group, One-way ANOVA test.

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Quercetin inhibits DNA damage responses to induce apoptosis via SIRT5/PI3K/AKT pathway in non-small cell lung cancer.

doi: 10.1016/j.biopha.2023.115071

Figure Lengend Snippet: Fig. 5. Quercetin inhibited NHEJ and HR pathways phosphorylation. Total proteins lysis and extraction after A549 and H1299 cultured with 0, 12.5, 50, and 200 μM quercetin for 24 h. In NHEJ pathways (A) the expression of p-DNA-PKcsS2056 (B, C), KU70 (D, E) and KU80 (F, G), and in HR pathways (H) the phosphorylation of p- ATRS428 (I, J), p-Chek1S345 (K, L), p-ATMS1981 (M, N) and Chek2T68 (O, P) were detected by western blot in both A549 and H1299 cells. And the results were measured by ImageJ and expressed as protein expression relative to GAPDH (mean ± S.D., n = 3). #p > 0.05, *p < 0.05, * *p < 0.01, * **p < 0.001 relative to values in the respective 0 μM group (B, p = 0.0048, R2 =0.7844, F=9.700; C, p = 0.0002, R2 =0.9042, F=25.17; D, p = 0.0054, R2 =0.7782, F=9.358; E, p = 0.0045, R2 =0.7879, F=9.908; F, p = 0.0037, R2 =0.7990, F=10.60; G, p = 0.0016, R2 =0.8379, F=13.79; I, p = 0.0034, R2 =0.8028, F=10.85; J, p = 0.0032, R2 =0.8066, F=11.12; K, p = 0.0010, R2 =0.8564, F=15.91; L, p = 0.0016, R2 =0.8379, F=13.79; M, p = 0.0044, R2 =0.7898, F=10.02; N, p = 0.0065, R2 =0.7676, F=8.806; O, p = 0.0030, R2 =0.0.8087, F=11.27; P, p = 0.0026, R2 =0.8166, F=11.87), One-way ANOVA test. The mRNA in A549 (Q) and H1299 (R) cells was extracted and reverse transcribed to cDNA for RT-qPCR analysis (mean ± S.D., n = 3). #p > 0.05, *p < 0.05, * *p < 0.01, * **p < 0.001, * ** *p < 0.0001 relative to values in the respective 0 μM group, One-way ANOVA test.

Article Snippet: Antibodies to γ-H2AXS139, p-ATRS428 (AP0676) and p-CDK1T161 (AP0324) were from Abclonal Technology (Abclonal, Wuhan, China); antibodies to KU70 (AF0300) and p-Chek1Ser345 (AF3008) were from Affinity Biosciences (Affinity, Jiangsu, China); antibodies to KU80 (PB9464) and p-DNAPKcsS2056 (BM4058) were from Boster Biological Technology (Boster, Wuhan, China); antibodies to p-ATMSER1981 (bsm-54103R) and pChek2Thr68 (ba-3721R) were from Biosynthesis Biotechnology (Bioss, Beijing, China); antibodies to p-PI3K (17366) and p-AKT (4060) were from Cell Signaling Technology (Danvers, MA, USA); antibodies to Caspase-3 (ab32042), Bax (ab32503), Bcl-2 (ab32124), SIRT5 (ab259967), GAPDH (ab9485) and β-tubulin (ab179511) were from Abcam (Abcam, Cambridge, UK).

Techniques: Phospho-proteomics, Lysis, Extraction, Cell Culture, Expressing, Western Blot, Reverse Transcription, Quantitative RT-PCR

XRCC5 was a target of miR‐326. (a, b) The expression of candidate targets was tested by quantitative reverse transcription‐polymerase chain reaction (qRT‐PCR) in breast cancer (BC) cells transfected with inhibitor NC or miR‐326 inhibitor. (c) The binding sites between miR‐326 and XRCC5 3′UTR predicted by TargetScan online software are shown. (d, e) Dual‐luciferase reporter assay was used to confirm the interaction between miR‐326 and XRCC5 3′UTR. (f) The mRNA expression of XRCC5 in BC tissues and adjacent normal tissues was examined by qRT‐PCR. (g) The protein expression of XRCC5 in MCF‐10A cells and both BC cells was determined using western blot (WB) analysis. (h) XRCC5 protein expression was tested by WB analysis in BC cells transfected with si‐NC, si‐RUSC1‐AS1, si‐RUSC1‐AS1 + inhibitor NC or si‐RUSC1‐AS1 + miR‐326 inhibitor. (i) The transfection efficiency of pc‐XRCC5 was assessed by measuring XRCC5 protein expression using WB analysis. (j) BC cells were transfected with miRNA NC, miR‐326 mimic, miR‐326 mimic + pc‐NC or miR‐326 mimic + pc‐XRCC5. WB analysis was used to detect XRCC5 protein expression. * p < 0.05.

Journal: Thoracic Cancer

Article Title: RUSC1‐AS1 promotes the malignant progression of breast cancer depending on the regulation of the miR‐326/XRCC5 pathway

doi: 10.1111/1759-7714.15035

Figure Lengend Snippet: XRCC5 was a target of miR‐326. (a, b) The expression of candidate targets was tested by quantitative reverse transcription‐polymerase chain reaction (qRT‐PCR) in breast cancer (BC) cells transfected with inhibitor NC or miR‐326 inhibitor. (c) The binding sites between miR‐326 and XRCC5 3′UTR predicted by TargetScan online software are shown. (d, e) Dual‐luciferase reporter assay was used to confirm the interaction between miR‐326 and XRCC5 3′UTR. (f) The mRNA expression of XRCC5 in BC tissues and adjacent normal tissues was examined by qRT‐PCR. (g) The protein expression of XRCC5 in MCF‐10A cells and both BC cells was determined using western blot (WB) analysis. (h) XRCC5 protein expression was tested by WB analysis in BC cells transfected with si‐NC, si‐RUSC1‐AS1, si‐RUSC1‐AS1 + inhibitor NC or si‐RUSC1‐AS1 + miR‐326 inhibitor. (i) The transfection efficiency of pc‐XRCC5 was assessed by measuring XRCC5 protein expression using WB analysis. (j) BC cells were transfected with miRNA NC, miR‐326 mimic, miR‐326 mimic + pc‐NC or miR‐326 mimic + pc‐XRCC5. WB analysis was used to detect XRCC5 protein expression. * p < 0.05.

Article Snippet: The sections were incubated with Ki67 antibody (1:50, Solarbio) or X‐ray repair cross‐complementing group 5 (XRCC5) antibody (1:100, Solarbio), followed by hatching with Goat Anti‐Rabbit IgG antibody (1:1000, GeneTex).

Techniques: Expressing, Reverse Transcription, Polymerase Chain Reaction, Quantitative RT-PCR, Transfection, Binding Assay, Software, Luciferase, Reporter Assay, Western Blot

MiR‐326 regulated breast cancer (BC) progression by targeting XRCC5. Breast cancer (BC) cells were transfected with miRNA NC, miR‐326 mimic, miR‐326 mimic + pc‐NC or miR‐326 mimic + pc‐XRCC5. The cell viability, number of colonies, number of migrated and invaded cells, cell cycle, apoptosis, and number of branches were determined using cell counting kit‐8 (CCK‐8) assay (a), colony formation assay (b), transwell assay (c, d), flow cytometry (e–g) and tube formation assay (h). (i) Western blot analysis was performed to examine E‐cadherin, N‐cadherin and fibronectin protein levels. * p < 0.05.

Journal: Thoracic Cancer

Article Title: RUSC1‐AS1 promotes the malignant progression of breast cancer depending on the regulation of the miR‐326/XRCC5 pathway

doi: 10.1111/1759-7714.15035

Figure Lengend Snippet: MiR‐326 regulated breast cancer (BC) progression by targeting XRCC5. Breast cancer (BC) cells were transfected with miRNA NC, miR‐326 mimic, miR‐326 mimic + pc‐NC or miR‐326 mimic + pc‐XRCC5. The cell viability, number of colonies, number of migrated and invaded cells, cell cycle, apoptosis, and number of branches were determined using cell counting kit‐8 (CCK‐8) assay (a), colony formation assay (b), transwell assay (c, d), flow cytometry (e–g) and tube formation assay (h). (i) Western blot analysis was performed to examine E‐cadherin, N‐cadherin and fibronectin protein levels. * p < 0.05.

Article Snippet: The sections were incubated with Ki67 antibody (1:50, Solarbio) or X‐ray repair cross‐complementing group 5 (XRCC5) antibody (1:100, Solarbio), followed by hatching with Goat Anti‐Rabbit IgG antibody (1:1000, GeneTex).

Techniques: Transfection, Cell Counting, CCK-8 Assay, Colony Assay, Transwell Assay, Flow Cytometry, Tube Formation Assay, Western Blot

Knockdown of RUSC1‐AS1 inhibited the tumorigenesis of breast cancer (BC) in vivo. (a) The tumor volume was measured every week. (b) After 4 weeks, the tumor was photographed and weighed. (c) RUSC1‐AS1, miR‐326 and XRCC5 expression levels were detected by quantitative reverse transcription‐polymerase chain reaction (qRT‐PCR). (d) XRCC5, E‐cadherin, N‐cadherin and fibronectin protein levels were determined by western blot analysis. (e) Immunohistochemical (IHC) staining was used to detect the Ki67 and XRCC5 positive cells in each group. * p < 0.05.

Journal: Thoracic Cancer

Article Title: RUSC1‐AS1 promotes the malignant progression of breast cancer depending on the regulation of the miR‐326/XRCC5 pathway

doi: 10.1111/1759-7714.15035

Figure Lengend Snippet: Knockdown of RUSC1‐AS1 inhibited the tumorigenesis of breast cancer (BC) in vivo. (a) The tumor volume was measured every week. (b) After 4 weeks, the tumor was photographed and weighed. (c) RUSC1‐AS1, miR‐326 and XRCC5 expression levels were detected by quantitative reverse transcription‐polymerase chain reaction (qRT‐PCR). (d) XRCC5, E‐cadherin, N‐cadherin and fibronectin protein levels were determined by western blot analysis. (e) Immunohistochemical (IHC) staining was used to detect the Ki67 and XRCC5 positive cells in each group. * p < 0.05.

Article Snippet: The sections were incubated with Ki67 antibody (1:50, Solarbio) or X‐ray repair cross‐complementing group 5 (XRCC5) antibody (1:100, Solarbio), followed by hatching with Goat Anti‐Rabbit IgG antibody (1:1000, GeneTex).

Techniques: Knockdown, In Vivo, Expressing, Reverse Transcription, Polymerase Chain Reaction, Quantitative RT-PCR, Western Blot, Immunohistochemical staining, Immunohistochemistry